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Effect of substituting lysine and P63 residues of HUα2 on supercoiling inducing ability.
The left panel outlines the assay carried out to determine the ability of HUα2 to induce negative supercoils in a nicked plasmid DNA. The middle panel shows the electrophoresis of the DNA products from the assay in the presence of the indicated concentrations of wild-type HUα2 protein or its variants. The right panel shows densitometric lane traces of the gel shown in the middle panel.
