Fig. 8.

Role of HU in regulating the orientation of the fimS switch

(A) The position of Hinfl restriction site when fimS switch is in ON or OFF phase. Red arrows represent the binding sites of the primers fimE-u1 and fimA-l1 used for polymerase chain reaction of fimS region. Solid red lines represent the PCR products and sizes of Hinfl restriction fragments in ON or OFF phase. IR inverted repeats

(B) Agarose geel after electrophoresis of Hinfl digestion reactions of the PCR products of chromosomal DNA of E. coli strains harboring the indicated deletion mutations in hupA and hupB genes and mutations in the hupA gene to introduce P63A amino acid substitution in the HUα subunit. Numbers correspond to genotypes in panel B. M DNA marker.

(C) Percentage amount of 520 bp fragment from total amount of 520 bp and 423 bp fragments. Graphical and error bars represent averages and standard deviation of three biological replicates respectively. Experiment was performed twice, with similar results. Statistically significant differences as determined by 1-way ANOVA with Dunnett’s multiple comparisons test. ns not significant; ** adjusted p-value 0.003; *** adjusted p-value <0.001.