Western blotting analysis showing the downregulation of ZO-1 (A) along with the corresponding Immunofluorescence images showing protein expression and distribution (B). Western blotting analysis showing the downregulation of Occludin (C) along with the corresponding Immunofluorescence images showing protein expression and distribution (D). TEER (E) and fluorescein permeability (F) demonstrating a significant decrease of BBB integrity. *p < 0.05, ** p < 0.01, ***p < 0.001, ****p < 0.0001 vs. control; +p < 0.05, ++p < 0.01 vs. gp120; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 vs. TS + gp120 co-treatment. n.s. = non statistical significance.