Table 1.
Comparison of biophysical properties of scFv/spFv bi- and tri-specifics.
| Molecule Name | Affinity (nM) |
% Aggregate |
Notes | ||
|---|---|---|---|---|---|
| Target 1 | Target 2 | 4°C | 40°C | ||
| BsAb-1 sc | 31.1 ± 22 | N/A | 27.2 | 21.4 | 2 wk @ 150 mg/ml |
| BsAb-1 sp | 11.6 ± 6.9 | N/A | 0 | 1.4 | 2 wk @ 142 mg/ml |
| BsAb-2 sc | 178 ± 61 | N/A | 15.4 | 17.4 | 2 wk @ 150 mg/ml |
| BsAb-2 sp | 177 ± 90 | N/A | 0 | 1.2 | 2 wk @ 150 mg/ml |
| BsAb-3 sc | 104 ± 15 | N/A | 0.9 | 29.6 | 2 wk @ 152 mg/mL |
| BsAb-3 sp | 135 ± 21 | N/A | 0.7 | 2.4 | 2 wk @ 155 mg/mL |
| BsAb-4 sc | 134 ± 62 | N/A | 0 | 17.9 | 2 wk @150 mg/mL |
| BsAb-4 sp | 110 ± 48 | N/A | 0 | 0.4 | 2 wk @ 150 mg/mL |
| TsAb-1 sc, sc | 0.169 | 235.9* | 15 | >40.0 | 4 wk @ 120 mg/ml |
| TsAb-1 sp, sp | 0.184 | 69.8* | <6 | <5.0 | 4 wk @ 120 mg/ml |
BsAb: bispecific antibody (
); TsAb: trispecific antibody (
), where 1 and 2 indicate targets 1 and 2. The sc/sp indicate format (scFv/spFv) of the single chain moiety. All affinity values by SPR. *cell binding EC50. Values for the scFv/spFv moieties only are given.