Resources table	SOURCE	IDENTIFIER
Bacterial and virus strains
E.coli XL10-GOLD KanR Ultracompetent Cells	Agilent	200317
E.coli DH10EMBacY Competent Cells	Geneva Biotech	DH10EMBacY
Chemicals, peptides, and recombinant proteins
Deuterium Oxide 99.9%	Sigma	151882
Guanosine 5′-diphosphate (GDP) sodium salt hydrate	Sigma	G7127-100MG
Guanosine 5′-triphosphate (GTP) sodium salt hydrate	Sigma	G8877-250MG
Sodium deoxycholate	Sigma	D6750
Polyoxyethylene (10) lauryl ether	Sigma	P9769
CHAPS, Molecular Biology Grade	EMD Millipore	220201
Phosphatidylserine (Porcine Brain)	Avanti	840032C
Phosphatidylethanolamine (Egg yolk)	Sigma	P6386
Cholesterol	Sigma	47127-U
Phosphatidylcholine (Egg yolk)	Avanti	840051C
Phosphatidylinositol-4,5-bisphosphate (Porcine Brain)	Avanti	840046
Sphingomyelin (Egg yolk)	Sigma	S0756
1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)	Avanti	850375C
1,2-dioleoyl-sn-glycero-3-phospho-L-serine (18:1, DOPS)	Avanti	840035C
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidomethyl)cyclohexane-carboxamide] (18:1 MCC-PE)	Avanti	780201C
10 mg/mL beta casein solution	ThermoFisher	37528
10x PBS [pH 7.4]	Corning	46-013-CM
glucose oxidase from Aspergillus niger (225 U/mg)	Biophoretics	B01357.02
catalase	Sigma	C40-100MG Bovine Liver
Trolox	Cayman Chemicals	10011659
Dyomics 647 maleimide dye	Dyomics	647P1-03
Coenzyme A	Sigma	C3019
Sulfuric acid	Sigma	58105-2.5L-PC
C-Flat 2/2-T grids	FisherScientific	50-192-8327
Critical commercial assays
Transcreener ADP2 FI Assay (1,000 Assay, 384 Well)	BellBrook Labs	3013-1K
Deposited data
PDB coordinate file for p110γ-NB7 structure	PDB	8DP0
EM density file for p110γ-NB7 complex	EMD	EMD-27627
HDX-MS and phosphorylation proteomics data	PRIDE	PXD040765
Oligonucleotides
Fwd primer for amplifying KD of PKCβII GTATTTTCAGGGCgccggtaccACGACCAACACTGTCTCCAAATTTG	Sigma	MR51F
Rvs primer for amplifying KD of PKCβII gactcgagcggccgcTTATAGCTCTTGACTTCGGGTTTTAAAAATTCAG	Sigma	MR51R
Fwd primer for amplifying N term of PKCβII CCATCACggatctggcggtagtATGGCTGACCCGGCTGCG	Sigma	MR52F
Rvs primer for amplifying N term of PKCβII GCCCTGAAAATACAGGTTTTCCTTTTCTTCCGGGACCTTGGTTCCC	Sigma	MR52R
Fwd primer for adding stop codon to PKCβII AGTCAAGAGCTAAgcggccgctcgagtctagagcctgc	Sigma	MR56F
Rvs primer for adding stop codon to PKCβII gactcgagcggccgcTTAGCTCTTGACTTCGGGTTTTAAAAATTCAG	Sigma	MR56R
Recombinant DNA
pMultiBac-Gβ1/Gγ2	PMID:34452907	pOP737
pACEBac1-hsp110γ	PMID:34452907	MR30
pMultiBac-hsp110γ-ssp101	PMID:34452907	MR22
pMultiBac-hsp110γ-mmp84	PMID:34452907	MR24
pFastBac HRas G12V	PMID:34452907	BS9
biGBac hsp110γ/ybbr-hsp84	PMID:36842083	HP28
biGBac hsp110γ/ybbr-hsp101	PMID:36842083	HP29
his6-GST-PrescissionProtease-SNAP-RBD(K65E)	PMID:34452907	pSH936
his6TEV-HRas(1-184aa) C118S, C181S	PMID:34452907	pSH414
his6-Gγ2, SNAP-Gβ1 (DUAL FastBac)	PMID:34452907	pSH651
pACEBAC-PKCβII (internal tev cleavage site)	This paper	pMR56
pFASTBac p110α	PMID: 28515318	pOV1181
pFASTBac p110β	PMID: 28515318	pOV1182
pFASTBac p110δ	PMID: 28515318	pOV1183
pFASTBac p85β	This paper	EX21
Software and algorithms
COOT-0.9.4.1	CCP4	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Phenix-1.19.1	Open source	https://www.phenix-online.org/
PDBePISA (Proteins, Interfaces, Structures and Assemblies)	EMBL-EBI	https://www.ebi.ac.uk/pdbe/pisa/pistart.html
ESPript 3.0	Robert et al NAR 2014	https://espript.ibcp.fr
HDExaminer	Sierra Analytics	http://massspec.com/hdexaminer
GraphPad Prism 7	GraphPad	https://www.graphpad.com
PyMOL	Schroedinger	http://pymol.org
Compass Data Analysis	Bruker	https://www.bruker.com
ChimeraX	UCSF	https://www.rbvi.ucsf.edu/chimerax/
ImageJ/Fiji	ImageJ	https://imagej.net/software/fiji/
Nikon NIS elements	Nikon	https://www.microscope.healthcare.nikon.com/products/software/nis-elements
cryoSPARC v.3.3.2	Structura Biotechnology	https://cryosparc.com/
Other
Sf9 insect cells for expression	Expression Systems	94-001S
Insect cell media	Expression Systems	96-001-01
Hellmanex III cleaning solution	Fisher	14-385-864
6-well sticky-side chamber	IBIDI	80608