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Primary antibodies used for immunofluorescence
| Protein | Species | Dilution | Catalog # | Vendor |
|---|---|---|---|---|
| ISL1 | Mouse | 1:200 | 39.4D5 | DSHB |
| SOX2 | Rabbit | 1:200 | 3579S | Cell Signaling Technology |
| NANOG | Goat | 1:100 | AF1997 | R&D Systems |
| HAND1 | Goat | 1:200 | AF3168 | R&D Systems |
| GATA3 | Rabbit | 1:800 | 5852S | Cell Signaling Technology |
| TFAP2C | Mouse | 1:150 | SC-12762 | Santa Cruz Biotechnology |
| OCT3/4 | Mouse | 1:400 | 611202 | BD Biosciences |
| pSMAD1/5/9 | Rabbit | 1:100 | 13820S | Cell Signaling Technology |
