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[Preprint]. 2023 Apr 11:2023.04.10.536247. [Version 2] doi: 10.1101/2023.04.10.536247

Figure 2. Glycosylase activity enhances telomeric 8oxoG-induced cytoplasmic DNA.

Figure 2.

(A) Representative IF-FISH image of cytoplasmic DNA species stained by DAPI (blue) containing telomeres (green), γH2AX (red) or 53BP1 (purple) in WT cells 24h after recovery from 20 min DL.

(B) Quantification of cytoplasmic chromatin fragments (CCF) 24h after recovery from 20 min DL from panel A. Data are mean ± s.d. from 4 independent experiments, of at least 400 nuclei counted per experiment; 2-way ANOVA; *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

(C) Quantification of the percent of CCFs from panel (A) positive for DDR markers γH2AX or 53BP1 for the indicated cell lines. Data are mean ± s.d. from 3 independent experiments; 2-way ANOVA; **p ≤ 0.01, ****p ≤ 0.0001.

(D) Quantification of the percent of chromatin bridges 24h after recovery from 20 min DL. Data are mean ± s.d. from 3 independent experiments, of at least 1,000 nuclei counted per experiment; 2-way ANOVA; *p ≤ 0.05, **p ≤ 0.01.