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[Preprint]. 2023 Apr 11:2023.04.10.536247. [Version 2] doi: 10.1101/2023.04.10.536247

Figure 4. BER intermediates promote telomeric 8oxoG-induced senescence.

Figure 4.

(A) Immunoblot of poly-ADP ribose (PAR) in WT BJ FAP-TRF1 treated with 10 μM PARG inhibitor PDD 00017272 (PARGi), 10 μM PARP1 inhibitor AZD2281 (Olaparib - PARPi) and with DL for the indicated times. α-tubulin used as a loading control.

(B) Immunoblot of PAR in cell lines treated with 10 μM PARGi and with 20 min DL. α-tubulin used as a loading control.

(C) Percent β-galactosidase positive cells treated with 20 min DL with or without 0.5 μM Olaparib (PARPi). Data are mean ± s.d of 3 independent experiments; 2-way ANOVA; ***p ≤ 0.001, ****p ≤ 0.0001.