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[Preprint]. 2023 Apr 11:2023.04.10.536247. [Version 2] doi: 10.1101/2023.04.10.536247

Figure 5. Both BER and replication stress activate PARylation after 8oxoG damage.

Figure 5.

(A) Schematic of experiments for PAR detection by WB (B) and telomere IF-FISH (C,D) in unsynchronized (cells grown with 10% FBS (+FBS)) and quiescent (cells grown with 0.1% FBS (−FBS)) WT cells.

(B) Immunoblot of PAR unsynchronized (+FBS) or quiescent (−FBS) WT cells treated with 10 μM PARGi and with no treatment or after 20 min DL. α-tubulin used as loading control.

(C) Representative IF-FISH images showing PAR (red) colocalizing with telomeres (green) by telo-FISH in + or −FBS WT cells with no treatment or after 20 min DL. Yellow arrowheads point to colocalization of PAR with telomeres.

(D) Quantification of the number of PAR foci colocalizing with telomeres per nuclei analyzed; each dot represents a nucleus. Data are mean ± s.d; ordinary one-way ANOVA with Tukey’s multiple comparisons test; NS = not significant (show only for untreated and DL comparisons); ****p < 0.0001.