Figure 6. OGG1 loss sensitizes cells to chronic telomere damage while MUTYH loss promotes resistance.
(A-B) Population doubling (PD) over 24 days of untreated cells (solid line) and cells treated with DL for 20 min each day except every 4th day of harvest (dotted line). Data are mean ± s.d. from 3 independent experiments.
(C). Simple linear regression showing the slope (PD/Day) of cells treated 18 times with 20 min DL. Error bars represent the 95% confidence intervals. </p/>(D) Percent β-galactosidase positive cells exposed for 18 times to 20 min DL over 24 days. Data are mean ± s.d. from 2 independent experiments; 2-way ANOVA; NS = not significant (shown only for untreated to DL comparisons);* p ≤ 0.05, **p ≤ 0.01, ***P ≤ 0.001.
(E) Quantification of CCF/MN foci 24 h after recovery from last exposure to 20 min DL (x18). Data are mean ± s.d. from 3 independent experiments, of at least 400 nuclei scored per each experiment; 2-way ANOVA; NS = not significant (show only for untreated to DL comparisons), *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001.
(F) Quantification of the number of bridges per 100 DKO cells counted. Data are mean ± s.d. from 2 independent experiments, of at least 1,000 nuclei counted per experiment; 2-way ANOVA; NS = not significant (shown only for untreated to DL comparisons); **p ≤ 0.01, ****P ≤ 0.0001.
(G) Representative image of micronuclei and chromatin bridge in DKO cells after 24h from last of 18 exposures.