chemerin knockout increased pro-inflammatory cytokines in the heart of HFD mice
Rarres2−/− and Littermate WT mice were subjected to high-fat diet feeding for 20 weeks.
(A) Serum chemerin level was detected by ELISA in both Rarres2−/− and Littermate mice, n = 6.
(B) The RT-qPCR analysis of pro-inflammatory cytokines in the heart between Rarres2−/− and littermate WT mice, n = 3.
(C) Western blots and its quantification of p-P38, P38, and p-P65, P65 in the heart. The HSP90 was used as a loading control (n = 6). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.