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. 2023 Mar 24;26(4):106495. doi: 10.1016/j.isci.2023.106495

Figure 5.

Figure 5

Chemerin knockout causes metabolic substrate inflexibility and cardiac metabolic dysfunction in HFD mice

Rarres2−/− and Littermate WT mice were subjected to high-fat diet feeding for 20 weeks. Calorimetric parameters about male Rarres2−/− and WT littermate mice were individually placed in the metabolic cages for 2-3 days of measurement; (A) oxygen consumption; (B) carbon dioxide production; (C) Respiratory exchange ratio (RER).

(D) Daily locomotor activity, n = 5. ∗p < 0.05, (E-I) Serum levels of NEFA, TG, TC, HDL-C, and No HDL-C in Rarres2−/− and littermate mice, n = 6.

(J) Metabolic Heatmap and hierarchical clustering of lipid metabolites in the heart of Rarres2−/− and littermate mice.

(K)Top metabolic pathways enriched in Rarres2−/− mice identified by Molecular Pathway Level Analysis (false discovery rate<0.05).

(L) mRNA expression levels of lipogenic genes, n = 6.

(M) Representative Western blots and their quantification of PPARγ and SCD1. The HSP90 was used as a loading control (n = 7). ∗p < 0.05, ∗∗p < 0.01.