Figure 6.

Chemerin supplementation restores lipid overload-induced lipotoxicity and inflammation in cardiomyocytes

Neonatal mouse primary cardiomyocytes (NMCMs) were first exposed to a control medium, high palmitate medium (HP) for 12h, and then treated with either 200 ng/ml chemerin or si-CMKLR1 for another 24h.

(A) Cells were stained with Oil red O.Scale bar = 50μm.

(B) Representative Western blots and its quantification of lipids metabolism enzymes (e.g., ACC, SCD1, and PPARγ), n = 4.

(C) Representative Western blots and its quantification of phosphorylation AKT (p-AKT ser 473). The HSP90 was used as a loading control (n = 3).

(D and E) mRNA expressions of pro-inflammatory cytokines (e.g., TNFα and IL-1β), n = 3.