Fig. 3. GAP43 interacts with CB1R in MC axon terminals of the DG.

PLA experiments were performed in hippocampal sections from 3-month-old mice of different genotypes. CB₁R-GAP43 complexes are shown as PLA-positive red dots. Nuclei are stained with DAPI (blue). a Representative images of DG-IML sections from Cnr1^fl/fl, GABA-Cnr1^−/−, Glu-Cnr1^−/−, and full Cnr1^−/− mice. Arrowheads point to some of the complexes. Inset magnifications are included for each genotype. Quantification of PLA-positive dots per field is shown (right, means ± SEM; Cnr1^fl/fl n = 5 mice, GABA-Cnr1^−/− n = 5 mice, Glu-Cnr1^−/− n = 5 mice, Cnr1^−/− n = 6 mice; one-way ANOVA with Tukey’s multiple comparisons test). b Representative images of DG-IML sections from Stop-Cnr1, GABA-Cnr1-RS, Glu-Cnr1-RS, and Cnr1-RS mice. Arrowheads point to some of the complexes. Inset magnifications are included for each genotype. Quantification of PLA-positive dots per field is shown (right, means ± SEM; n = 7 mice per group; one-way ANOVA with Tukey’s multiple comparisons test). Source data are provided as a Source data file.