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. 2023 Apr 21;14:2303. doi: 10.1038/s41467-023-38026-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Timeline of the experiments. Vehicle or THC (10 mg/kg, i.p.; 1 injection) was administered to 3-month-old Glu-Gap43^−/− mice and their corresponding Gap43^fl/fl littermates. Kainic acid (KA; 30 mg/kg, i.p.; 1 injection) was administered 15 min later, and behavioral score (b–d) or hippocampal EEG recording (e–h) was monitored continuously for 120 min. b Behavioral scoring of seizures using a modified Racine scale (means ± SEM; number of mice in parentheses; the shaded area indicates all the time points at which p < 0.05 by two-way ANOVA with Sidak’s multiple comparisons test;). c Integrated seizure severity, expressed as normalized percentage from Gap43^fl/fl/Vehicle group (means ± SEM; Gap43^fl/fl/Vehicle n = 17 mice, Gap43^fl/fl/THC n = 18 mice, Glu-Gap43^−/−/Vehicle n = 17 mice, Glu-Gap43^−/−/THC n = 16 mice; two-way ANOVA with Tukey’s multiple comparisons test). d Latency to seizures (means ± SEM; Gap43^fl/fl/Vehicle n = 17 mice, Gap43^fl/fl/THC n = 18 mice, Glu-Gap43^−/−/Vehicle n = 17 mice, Glu-Gap43^−/−/THC group n = 16 mice; two-way ANOVA with Tukey’s multiple comparisons test). e EEG recordings of representative Glu-Gap43^−/− mice treated with vehicle (top) or THC (bottom). Epileptic-like spikes appeared after KA injection (insets: detail of individual spikes). The corresponding sonograms (frequency spectrum along recording time) are shown below each recording. f Latency to EEG seizure onset (means ± SEM, n = 10 mice per group; two-way ANOVA with Tukey’s multiple comparisons test). g Interictal frequency (means ± SEM; Gap43^fl/fl/Vehicle n = 9 mice, Gap43^fl/fl/THC n = 9 mice, Glu-Gap43^−/−/Vehicle n = 10 mice, Glu-Gap43^−/−/THC n = 10 mice; two-way ANOVA with Tukey’s multiple comparisons test). h Average spike duration (means ± SEM; Gap43^fl/fl/Vehicle n = 9 mice, Gap43^fl/fl/THC n = 9 mice, Glu-Gap43^−/−/Vehicle n = 10 mice, Glu-Gap43^−/⁻/THC n = 10 mice; n.s. by two-way ANOVA with Tukey’s multiple comparisons test). Source data are provided as a Source data file.