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. 2023 Apr 21;13:6528. doi: 10.1038/s41598-023-33848-y

Figure 5.

Figure 5

HIF-1α siRNA mitigates autophagosome accumulation and lipid deposition in irinotecan (IR)-treated HepG2 cells. (A) Western blot analysis of BNIP3, LC3-II, Beclin1 and p62 protein levels in HepG2 cells. (B) Densitometric quantification of BNIP3, LC3-II Beclin1 and p62. (C) IR exacerbated TG content in HepG2 cells and co-treated with HIF-1α siRNA decreased cellular TG content. The blots were cut prior to hybridisation with indicated primary antibodies. Data are presented as mean ± SD (n = 3), *P < 0.05 and **P < 0.01 compared with normal control group, #P < 0.05 and ##P < 0.01 compared with 20 μM IR-treated group.