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. 2023 Apr 21;13:7. doi: 10.1038/s41387-023-00235-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Alpha diversity calculated on rarefied ASV data, p value represents the result of Wilcoxon test; B 2D PCA scores plot on genera level. The explained variance of each component is included in the axis labels. The large points represent the centroids of each group. C Biomarker bacterial genera. Prior to all calculations, data were clr transformed. Biomarkers were generated from univariable discriminant analysis (FDR ≥0.1), with effect size estimated by Cliff’s delta with a 95% confidence interval. A, time point prior to intervention; B, time point post-intervention. Core genera (bold) are defined by the condition abundance >0.05% and prevalence >75% at least in one group. Genera marked by * are confirmed butyrate producers, and genera marked by (*) are potential butyrate producers. ASV, amplicon sequence variant.