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. 2023 Apr 21;14:2285. doi: 10.1038/s41467-023-37674-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A NMP perfusate analysis workflow and applied methods. B Cell viability testing prior to flow cytometry (n = 3 pre, at 1 h NMP and at the end of NMP) showed only a very small percentage of non-viable cells in the perfusate. C Absolute CD45⁺ leukocyte amounts for main immune cell subtypes in total circulating perfusate (mean ± SEM) and D composition at 1 h NMP. E, F Dynamic change of total CD45⁺ leukocytes and main subtypes during NMP. G Dynamic change of CD3⁺ T cell subtypes (proportions) over perfusion time. Graphs show the marginal effects. The values are estimated using linear regression analysis. The p-values refer to the change over time. The least-squares means computed using a linear model are shown together with the 95% CI. N = 26 biologically independent samples. Source data are provided as a Source Data file. NK cells natural killer cells, NKT cells natural killer T cells, MAIT cells mucosa-associated invariant T cells.