In vivo analysis of osteochondral regeneration using in situ self-assembled organoids, including MRI and macroscopic evaluation of repair tissues. (A) Schematic of the in vivo experiment in beagle dogs. (B) MRI evaluation of the repair tissue at 3 and 6 months post-surgery. Red arrows indicate the region of repair tissue. (C, D) WORMS scoring of repair tissue at 3 and 6 months. (E) Macroscopic evaluation of repair tissue at 3 and 6 months. Red circles indicate the defect area. (F) Heatmap of ICRS macroscopic scores. (G) ICRS macroscopic scores of repair tissue at 3 and 6 months. (*p < 0.05, **p < 0.01, ***p < 0.005, n. s. represents no significant difference, n = 3).