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. 2023 Apr 21;14:2316. doi: 10.1038/s41467-023-37994-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Wapl protein expression in ex vivo sorted Rag2^–/– pro-B (gray) and wild-type pre-B (black) cells from the bone marrow, as determined by immunoblot analysis of whole-cell extracts with antibodies detecting Wapl or Tbp (loading control). Marker proteins of the indicated size (in kilodaltons, kDa) are shown to the right. A non-specific band is indicated by an asterisk. One of 5 independent experiments is shown, and the quantification of all immunoblot experiments is indicated to the right. Statistical data are shown as mean values with SEM and were analyzed with the Student’s t-test (unpaired and two-tailed). b Comparison of the frequency distribution of intrachromosomal contacts between pro-B and pre-B cells of Wapl^+/+ mice. The frequency distribution of intrachromosomal contacts was determined as a function of the genomic distance using logarithmically increased genomic distance bins, based on the Hi-C data of Wapl^+/+ pre-B cells (black, this study) and published Hi-C data of short-term cultured Wapl^+/+ pro-B cells (gray)²⁰. c Hi-C contact matrices of chromosome 1, determined for Wapl^+/+ pro-B and Wapl^+/+ pre-B cells, were plotted at a 500-kb bin resolution with Juicebox⁷⁴. The maximum pixel intensity is indicated below (red square). d Hi-C contact matrices of a zoomed-in region on chromosome 16 (mm9; 22,500,000–28,000,000), shown for Wapl^+/+ pro-B and pre-B cells. Black dots indicate loop anchors identified with Juicebox⁷⁴. e Distribution of the loop length (in kb) in Wapl^+/+ pro-B (gray) and pre-B (black) cells. White lines indicate the median and boxes represent the middle 50% of the data. Whiskers denote all values of the 1.5× interquartile range. The median loop length is 375 kb in Wapl^+/+ pro-B cells and 250 kb in Wapl^+/+ pre-B cells. f Comparison of the frequency distribution of intrachromosomal contacts between Wapl^+/+ pre-B cells (black) and Wapl^{∆P1,2/∆P1,2} pro-B cells (green)²⁰, as described in (b). Source data are provided in the Source Data file.