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. 2023 Apr 21;6:440. doi: 10.1038/s42003-023-04826-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a RAB35 protein levels in the whole mouse brain during the embryonic (E15.5 and E17.5) and postnatal (P2, P6, P14, and P21) periods. b Quantification of RAB35 protein levels in the whole mouse brain. Band intensities of RAB35 were normalized against those of actin (n = 3 per the indicated stage). c RAB35 protein levels in the hippocampus during the embryonic (E15.5 and E17.5) and postnatal (P2, P6, P14, and P21) periods. d Quantification of RAB35 protein levels in the mouse hippocampus (n = 3 per stage). e X-gal staining of sagittal sections from E15 and P1 Rab35^geo/+ mouse brains. Scale bars, 200 μm. f RAB35 protein levels in 3-month-old mouse brain subregions. Ob olfactory bulb, Cx cerebral cortex, Hp hippocampus, Th thalamus, Cb cerebellum. g X-gal staining of a sagittal section of a 3-month-old Rab35^geo/+ mouse brain. Scale bars, 500 μm. h, i Representative maximum intensity projection images of 4-month-old Rab35^geo/+ mouse brain stained for β-galactosidase (green), NeuN or GFAP (magenta), and DAPI (blue). Scale bar; upper panel, 50 μm; lower panel, 20 μm. Data represent mean ± SEM.