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. Author manuscript; available in PMC: 2024 Apr 19.
Published in final edited form as: Neuron. 2023 Feb 13;111(8):1301–1315.e5. doi: 10.1016/j.neuron.2023.01.015

Figure 1. Social deprivation alters core properties of hippocampal astrocytes.

Figure 1.

(A) Schematic of Group Housing (SH) and Single Housing (SH) Paradigms.

(B) Imaging of Aldh1l1-GFP astrocytes and quantification of morphological complexity using Scholl analysis from prefrontal cortex, striatum, CA1, thalamus, and amygdala (n = 3 pairs of animals, at least 10 cells from each animal). Two-way ANOVA.

(C) Immunostaining for GFAP expression in prefrontal cortex, striatum, CA1, thalamus, and amygdala of Aldh1l1-GFP mice from GH and SH cohorts. Welch’s t-test. (n = 3 pairs of animals, 3 sections per animal)

(D) Behavioral results from GH and SH cohorts (n = 8-10 animals)

(E) Representative image showing expression of GCaMP6s in astrocytes and representative spontaneous Ca2+ of two-photon, slice imaging from the hippocampus of GH and SH mice quantification is derived from n = 19-20 cells from 3 pairs of animals. Welch’s t-test.

*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.