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. Author manuscript; available in PMC: 2024 Apr 19.
Published in final edited form as: Neuron. 2023 Feb 13;111(8):1301–1315.e5. doi: 10.1016/j.neuron.2023.01.015

Figure 2. TRPA1 expression is increased in astrocytes after social deprivation.

Figure 2.

(A) Serut analysis of single cell RNA-Seq (scRNA-Seq) of hippocampal astrocytes from a pair of GH and SH.

(B) KEGG pathway analysis of differentially expressed gene (DEGs) between GH and SH cohorts (cutoff for DEGs, adjusted p < 10−15, average log2 foldchange > 2).

(C) Immunostaining for TRPA1 expression in Aldh1l1-GFP mice from GH and SH cohorts (n = 16-22 cells from 3 pairs of animals). Welch’s t-test.

(D) Representative image showing expression of GCaMP6s in astrocytes and representative spontaneous Ca2+ of two-photon, slice imaging from the hippocampus of SH mice, treated with vehicle or HC030031 (HC); quantification is derived from n = 16 cells from 3 pairs of animals. Welch’s t-test.

*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.