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. Author manuscript; available in PMC: 2024 Apr 19.
Published in final edited form as: Neuron. 2023 Feb 13;111(8):1301–1315.e5. doi: 10.1016/j.neuron.2023.01.015

Figure 7. Knockdown of Best1 restores hippocampal circuit function after social deprivation.

Figure 7.

(A) Schematic of AAV-Cre and lentiviral-based shRNA approach for knocking down Best1 in hippocampal astrocytes and time line of experimental paradigm.

(B) Immunostaining for Best1 in hippocampal astrocytes from SH cohort after Best1 knockdown paradigm. (n = 3 pairs of animals) Welch’s t-test

(C) Representative traces measuring tonic GABA currents in CA1 pyramidal neurons in SH cohorts Cre plus scramble or Cre plus Best1 shRNA.

(D-F) Quantification of tonic GABA current (D), sIPSC amplitude (E), and sIPSC frequency (F) from the same cohorts and experimental conditions. Welch’s t-test

(G) Recording of Long-Term Potentiation (LTP) from the hippocampus in SH cohorts treated under control (Scramble) or Best1 knockdown (Best1 shRNA) genetic conditions. Welch’s t-test. *p < 0.05; ****p < 0.0001.