KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-GFP (1:1000) | Abcam | Cat# ab13970, RRID: AB_300798 |
| ouse anti-GFAP (1:1000) | EMD Millipore | Cat# MAB360, RRID: AB_11212597 |
| Rabbit anti-Sox9 (1:650) | EMD Millipore | Cat# 211-032-171, RRID: AB_2239761 |
| Guinea pig anti-GABA (1:200) | EMD Millipore | Cat# HAF016, RRID: AB_91011 |
| Rabbit anti-MAOB (1:200) | Proteintech | Cat# 12602-1AP, RRID: AB_2137273 |
| Mouse anti-mCherry (1:500 | Abcam | Cat# ab125096, RRID: AB_11133266 |
| Rabbit anti-TRPA1 (1: 250) | Abcam | Cat# ab58844, RRID: AB_945957 |
| Rabbit anti-BEST1 (1:250) | GeneTex | Cat# GTX33037, RRID: NA |
| Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor™ 488 (1:500) | Thermo Fisher | Cat# A11039, RRID: AB_2534096 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (1:500) | Thermo Fisher | Cat# A11036, RRID: AB_10563566 |
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (1:500) | Thermo Fisher | Cat# A11004, RRID: AB_2534072 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (1:500) | Thermo Fisher | Cat# A32723, RRID: AB_2633275 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (1:500) | Thermo Fisher | Cat# A21245, RRID: AB_2535813 |
| Goat anti-Guinea Pig IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 (1:500) | Thermo Fisher | Cat# A11075, RRID: AB_2534119 |
| Bacterial and virus strains | ||
| AAV2/9-pAAV-Gfap-Cre-P2A-TurboRFP | Ung et al., 2021 | NA |
| AAV2/9-pAAV-U6-Trpa1 sgRNA-Gfap-mcherry | This paper | NA |
| lenti-pSico-Maob shRNA (U6-loxP-CMV-EGFP-loxP-Maob shRNA) | Yoon et al., 2014 | NA |
| AAV2/9-CMV-LSL-mCherry-U6-SCR-SE | Kwak et al., 2020 | NA |
| AAV2/9-CMV-LSL-mCherry-U6-mBest1-sh-SE | Kwak et al., 2020 | NA |
| AAV2/9-GFAP-MAOB-GFP | This paper | NA |
| Experimental models: Organisms/strains | ||
| Ai96 (RCL-GCaMP6s) | JAX lab | RRID: IMSR_JAX:024106 |
| Aldh1l1-CreER | JAX lab | RRID: IMSR_JAX:029655 |
| Rosa26-LSL-Cas9 | JAX lab | RRID: IMSR_JAX:026175 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma | Cat# T5648 |
| Hoechst Nuclear Stain | Thermo Fisher | Cat# H3570 |
| APV | Tocris | Cat# 0106 |
| CNQX | Tocris | Cat# 1045 |
| GABA | Tocris | Cat# 0344 |
| BIC | Tocris | Cat# 0131 |
| Critical commercial assays | ||
| Neural Tissue Dissociation Kit | Miltenyl | Cat# 130-094-802 |
| Red Blood Cell Lysis Solution (10×) | Thermo Fisher | Cat# 130-094-183 |
| Chromium Single Cell Gene Expression 3v3.1 kit | 10X genomics | NA |
| NextSeq 500/550 High Output Kit v2.5 (75 Cycles) | Illumina | Cat# 20024906 |
| MACS Debris Removal Kit | Miltenyl | Cat# 130-109-398 |
| MACS Dead Cell Removal Kit | Miltenyl | Cat# 130-090-101 |
| MiniMACS Separator | Miltenyl | Cat# 130-042-102 |
| Dynabeads MyOne Silane Beads | Thermo Fisher | Cat# 37002D |
| Vectashield Antifade Mounting Medium | Vector Laboratories | Cat# H-1000 |
| Deposited data | ||
| Astrocyte single cell data from GH and SH | This paper | GSE222785 |
| Software and algorithms | ||
| ANYmaze | Stoelting Co. | NA |
| Versadat | Omnitech Electronics, Inc. | NA |
| 10X Cell Ranger (3.0.2) | 10X genomics | NA |
| Seurat (version 4.1.0) | Hao et al., 2021 | NA |
| DoubletFinder | McGinnis et al., 2019 | NA |
| SingleR | Aran et al. 2019 | NA |
| enrichR | Kuleshov et al., 2016 | |
| ClampFit 10.7 software | Molecular Devices LLC | NA |
| GECIquant | Venugopal et al., 2019 | NA |
| AQuA | Wang et al., 2019 | NA |
| Minianalysis | Blue cell | NA |
| SigmaPlot 13 | SYSTAT | NA |
| Prism 9 | Graphad | NA |
| Imaris | Oxford instruments | NA |