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. Author manuscript; available in PMC: 2023 Apr 22.
Published in final edited form as: Nat Struct Mol Biol. 2023 Feb 20;30(4):425–435. doi: 10.1038/s41594-023-00925-9

Extended Data Fig. 6 |. Co-pelleting assays of NuPODs with variants of CA tubes.

Extended Data Fig. 6 |

a, Schematic of the NuPOD-CA nanotube co-pelleting assay. b, Top: Supernatant (S) and pellet (P) fractions collected after co-pelleting Cy3-labelled NuPODs and A14C/E45C disulfide crosslinked CA tubes were analyzed on a 0.05% SDS–agarose gel. Fluorescence signals were used to detect the Cy3-labelled NuPODs. Black arrowheads indicate the NuPOD bands. Bottom: a plot showing the populations of Cy3-labelled NuPODs in the supernatant and pellet fractions. Data are plotted as mean±SD of three independent experiments (n = 3), with individual data points shown as black circles. c, Supernatant (S) and pellet (P) fractions from the co-pelleting assays analyzed by SDS-PAGE to detect the protein component of the NuPODs. The experiment was repeated three times with similar results. d, Negative-stain electron micrographs of supernatant (S) and pellet (P) samples from the co-pelleting assays. The experiment was repeated three times with similar results. Scale bar, 100 nm.