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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: Trends Neurosci. 2023 Mar 10;46(5):365–376. doi: 10.1016/j.tins.2023.02.005

Figure 3.

Figure 3.

Lipid nanoparticles (LNP), adeno-associated virus (AAV), and virus-like particles (VLP) as systems for delivery of CRISPR-Cas9 gene editing cargos. A. Molecular composition of a LNP for delivery of CRISPR-Cas9 gene editing cargos (see [34]). gRNA, guide RNA; PEG, polyethyleneglycol; PL, phospholipid. B. Examples of AAV constructs for gene editing. GFP, green fluorescent protein; ITR, inverted terminal repeat; NLS, nuclear localization sequence; prom, promoter; saCas9, Cas9 from staphylococcus aureus; spCas9, Cas9 from streptococcus pyrogenes; U6, an RNA polymerase II promoter. C. An example of a VLP containing CRISPR-Cas9 cargos. The viral gag protein contains the protease required for cleaving polyproteins into subunits and also provides structural stability to VLP. RBP, RNA-binding protein; RV, retroviral.