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. Author manuscript; available in PMC: 2024 May 1.
Published in final edited form as: J Immunol. 2023 May 1;210(9):1372–1385. doi: 10.4049/jimmunol.2200900

Figure 3. Endotoxemia-induced expression of APR factors is hepatic zone specific.

Figure 3.

(A-C) Fold enrichment of mRNA expression of periportal markers (A) Alb, (B) Ass1 and (C) Cyp2f2 n periportal hepatocytes isolated using digitonin-collagenase perfusion. Data were normalized to pericentral expression and expressed as mean ± SEM (N = 8–10 per isolation). *p < 0.05 vs. PC expression (D-E) Fold enrichment of mRNA expression of pericentral markers (C) Cyp2e1, and (D) Glul in pericentral (PC) hepatocytes isolated using digitonin-collagenase perfusion. Data were normalized to periportal expression and expressed as mean ± SEM (N = 8–10 per isolation). *p < 0.05 vs. PP expression (F) Representative Western blot of PEPCK, glutamine synthetase, and CYP2E1 on pericentral (PC), periportal (PP) and whole liver (W) lysates. On this representative image, isolations from six separate animals are shown (Two each for PC, PP and W). Total protein stain provided as loading control. (G-L) Fold change in mRNA expression of (F) Crp, (G) Saa-1, (H) Apcs, (I) Fga (J) Hp and (K) Lbp in pericentral (PC) and periportal (PP) hepatocytes isolated from endotoxemic (LPS 5 mg/kg, IP) mice. Data expressed as mean ± SEM (N = 4–6 per time point). Results of 2way ANOVA for interaction, zone and time provided. Results of multiple comparisons given. *p < 0.05 vs. unexposed zone-matched control. †p < 0.05 vs. PC hepatocytes isolated from similarly exposed animals. h = hours. (M) Representative Western blot for CRP and Cyp2e1 on pericentral (PC) and periportal (PP) cytosolic protein samples from endotoxemic (LPS 5 mg/kg, IP; 5 hours) mice. In this image, isolations from eight separate animals are shown (Four each for PC and PP). (N) Densitometric analysis of pericentral (PC) and periportal (PP) cytosolic CRP from mice exposed to sublethal endotoxemia (LPS 5 mg/kg, IP; 5 hours). Data normalized to total protein and expressed as mean ± SEM (N = 6–11 per time point). Results of 2way ANOVA for interaction, zone and time provided. Results of multiple comparisons given, with *p<.05 vs. unexposed zone-matched controls. †p<.05 vs. time-matched similarly exposed PC hepatocytes isolated from similarly exposed animals.