Fig. 4.

Female EC increased ROS production in response to AngII compared to male EC. A RNA-seq of differentially expressed genes associated with mitochondrial respiration were elevated in pooled female but not male HUVEC following AngII treatment (n = 3 samples per condition). B Representative images and quantification of ROS production, measured via Carboxy-H₂DCFDA (green), in female versus male pooled HUVEC, individual donor HUVEC, and individual donor HPAEC following 24 h of AngII treatment (n = 6 donors per sex; each datapoint averaged from 3 samples per donor per condition)