Fig. 6.

CYBB was upregulated in female, but not male EC, which may contribute to elevated ROS production in AngII-treated female cells. A CYBB gene expression in untreated pooled female and male HUVEC and one female and male HPAEC donor by RT-PCR. Data analyzed with a Mann–Whitney test. B Western blot of NOX2 and β-Actin, with quantification, in pooled HUVEC treated with scramble siRNA or CYBB siRNA for 6–48 h. Data analyzed using Kruskal–Wallis test. C Representative fluorescent microscopy images of carboxy-H₂DCFDA (green) as an ROS indicator in pooled female and male HUVEC following CYBB siRNA (or scramble siRNA) and ± 1 µM AngII treatment for 24 h. Scale = 100 µm. D ROS quantification following siRNA knockdown. Data analyzed using two-way ANOVA with Tukey’s correction for multiple comparisons *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Western blot and ROS intensity were normalized to untreated female cells