REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC anti-human CD45	BD Pharmingen	Cat: 555485; Isotype: Mouse IgG1, κ; RRID: AB_314400; LOT #3074786
APC/Cyanine7 anti-human CD184	Biolegend	Cat: 306528; Isotype: Mouse IgG2a, κ; RRID: AB_2565994; LOT#B321997
FITC anti-human FPR1	Biolegend	Cat: 391604; Isotype: Mouse IgG1, κ; RRID: AB_2728398; LOT#B252257
PE anti-human Leukotriene B4 Receptor/BLT1	Cayman Chemical	Cat: LS-A101254-100; Isotype: Polyclonal Rabbit; LOT#LAH0521011
PE/Cyanine7 anti-human CD16	Biolegend	Cat: 302016; Isotype: Mouse IgG1, κ; RRID: AB_314216; LOT#310460
Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 633	Invitrogen	Cat: A 21070; LOT#2160429
Goat anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor Plus 488	Invitrogen	Cat: A32723; LOT#VA297822 and #VJ308481
mouse anti-DNA-histone 1 monoclonal IgG2a	SigmaMilllipore	Cat: MAB3864; LOT#3560106; Isotype: Mouse IgG2a
Rabbit anti-human myeloperoxidase	Dako	Cat: A0398; LOT#20082378; Isotype: Rabbit IgG
Rabbit polyclonal anti-citrullinated-Histone H3 (Arg2/8/17)	abcam	Cat: ab5103; Isotype: Rabbit IgG; RRID:AB_304752; LOT#GR27606-1
Rabbit monoclonal anti-neutrophil elastase (clone EPR7479)	abcam	Cat: ab131260; Isotype: Rabbit IgGa; RRID:AB_11155265
Bacterial andvirusstrains
P. aeruginosa CF airway isolates used in this study	Hannover Medical School	See Table S1
Biological samples
Donkey serum	Sigma Aldrich	Cat: D9663-10ML; LOT#SLBL4004V
Human AB Serum	Institute of Transfusion Medicine and Transplant Engineering (Hannover Medical School)	N/A
Chemicals, peptides, and recombinant proteins
AMPure XP Beads	Beckman Coulter	Cat: A63881
Albumin fraction V	Carl Roth	Cat: CP84.2; LOT#380298758
Aurion BSA-c	Fisher scientific	Cat: 50-248-05; LOT#50512/3
DiffQuick – Haema QuickStain	Labor + Technik Eberhard Lehmann	LT 005
Dimethyl sulfoxide	Carl Roth	Cat: 4720.1; CAS 67-68-5
Gelatin from cold water fish skin	Sigma Aldrich	Cat: G7041-100G; LOT#SLCC7086
GelRed	Linaris Biologische Produkte	Cat: 41003
Glutaraldehyde	Sigma-Aldrich	Cat: G5882; CAS 111-30-8
GoTaq Long PCR Master Mix	Promega	Cat: 114021
Hoechst/bisBenzimide H 33342 trihydrochloride	Sigma Aldrich	Cat: G7041-100G; LOT#BCBL5830V
Lead citrate	Sigma-Aldrich	Cat: 15326; CAS 6107-83-1
Lipopolysaccharide (P. aeruginosa serotype 10)	Sigma Aldrich	Cat: L7018-10MG
NEmo-2 FRET reporter	SiChem	Cat: SC-0201; Lot# SC-0201-003_P-125_3-IV.2
Osmium tetroxide	Sigma-Aldrich	Cat: O5500-250MG; CAS 20816-12-0
Paraformaldehyde 16% EM grade	Science Services	Cat: E15710-250
Poly-L-lysine	Sigma Aldrich	Cat: P4707; LOT#RNBJ8041
Polymorphprep	Progen	Cat: 1114683; LOT#00121
ProLong Gold antifade mountant	Invitrogen	Cat: P36930; LOT#2273617
Saponine	Serva Electrophoresis	Cat: 34655.01; CAS: 8047-15-2
Sivelestat sodium salt hydrate	Sigma Aldrich	Cat: S7198-5MG; LOT# 0000044591
Sodium cacodylate trihydrate	Sigma-Aldrich	Cat: 20840; CAS 6131-99-3
Sodium periodate	Sigma-Aldrich	Cat: S1878; CAS 7790-28-5
Unconjugated gold colloid 5 nm	Ted Pella	Cat: 15702-20
Unconjugated gold colloid 10 nm	Ted Pella	Cat: 15703-20
Triton X 100	Carl Roth	Cat: 3051.3; CAS: 9036-19-5
Tween 20	Sigma Aldrich	Cat: P1379-100 ml; LOT#SLCH7513
Uranyl acetate	Ted Pella	Cat: 19481; CAS: 541-09-3
Critical commercial assays
Cytotoxicity Detection Kit	Sigma Aldrich	Cat: 11644793001
Epoxy Embedding Medium Kit	Sigma-Aldrich	Cat: 45359
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit	Invitrogen	Cat: L34957
NEBNext Ultra II DNA Library Kit for Illumina	New England Biolabs	Cat: E7645L
NEBNext Multiplex Oligos for Illumina (Set 1)	New England Biolabs	Cat: E7335L
NEBNext Multiplex Oligos for Illumina (Set 2)	New England Biolabs	Cat: E6442S
NEBNext Multiplex Oligos for Illumina (Set 3)	New England Biolabs	Cat: E6444S
NextSeq 500/550 Mid Output kit v2.5 (300 cycles)	Illumina	Cat: 20024905
Qubit dsDNA HS Assay Kit	ThermoFisher Scientific	Cat: Q32854
Deposited data
Anonymous patient data	This work	see Table S1
P. aeruginosa PA14 genome	NC_002516.2	Database: https://www.ncbi.nlm.nih.gov/genome/?term=Pseudomonas+aeruginosa+PA14
Oligonucleotides
412 primer sequences	This work	Mendeley Data: https://data.mendeley.com/datasets/c87p4pytt9/draft?a=689a644e-37cc-448d-b1ef-0c16e6b2370d
Software and algorithms
FlowJo v10.6.1.	TreeStar	https://www.flowjo.com/
GraphPad Prism 8	GraphPad Software	https://www.graphpad.com/
R 3.4.0.	R	https://r-project.org
Pipeline of evaluation of amplicon sequences (PIASneutro)	This work	Database: https://data.mendeley.com/datasets/c87p4pytt9/draft?a=689a644e-37cc-448d-b1ef-0c16e6b2370d