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. 2023 Mar 26;72:101713. doi: 10.1016/j.molmet.2023.101713

Figure 6.

Figure 6

2-AGP impairs excitatory transmission to orexin neurons. (A) Representative traces of mEPSCs recorded in OX-eGFP neurons before (black) and after 2-AGP (250 nM) (blue). (B) Effect of 2-AGP on the mEPSCs amplitude in n = 6 neurons from 3 mice; p = 0.1, Two-tailed paired Wilcoxon signed-rank test. (C) Effect of 2-AGP on the frequency of mEPSCs in n = 6 neurons from 3 mice; ∗p < 0.05, Two-tailed paired Wilcoxon signed-rank test. (D) Representative traces of mEPSCs recorded in OX-eGFP neurons preincubated with AM095 (10 μM, green trace) and after 2-AGP treatment (250 nM, blue trace). (E) Plot of the mEPSCs amplitude in AM095 and AM095+2-AGP; n = 6 neurons from 3 mice; p = 0.75, Two-tailed paired Wilcoxon signed-rank test. (F) Plot of the mEPSC frequency in AM095 and AM095+2-AGP; n = 6 neurons from 3 mice: p = 0.62, Two-tailed paired Wilcoxon signed-rank test. (G) Representative traces of eEPSCs evoked in OX-eGFP neurons using extracellular stimulation before (black circles) and after 2-AGP (250 nM) (blue squares), P1 and P2 represent the first and the second eEPSCs evoked in an interspike interval of 10 ms. (H) Amplitude of the first eIPSC (P1) before and after 2-AGP (250 nM); n = 6 neurons from 3 mice; ∗p < 0.05, Two-tailed paired Wilcoxon signed-rank test. (I) Ratio between two consecutive eIPSCs evoked between 10 ms and 1sec in control (black circle) and after 2-AGP (blue square) (n = 6 neurons from 3 mice; ∗p < 0.05, Two1005 tailed paired Wilcoxon signed-rank test). The ratio is plotted as a function of the inter-spike interval on a logarithmic scale. (J) Representative traces of paired-pulse at 10 ms in AM095 (10 mM) (green trace) and AM095 + 2-AGP (250 nM) (blue trace) in OX-eGFP neurons using extracellular stimulation. P1 and P2 represent the first and the second eEPSCs evoked in an interspike interval of 10 ms. (K) Amplitude of the first eIPSC (P1) in AM095 and after 2-AGP (250 nM); n = 6 neurons from 3 mice. (L) Ratio between two consecutive eIPSCs evoked between 10 ms and 1sec in AM095 (green circles) and after the treatment with 2-AGP (blue squares). The ratio is plotted as a function of the inter-spike interval on a logarithmic scale. n = 6 neuron from 3 mice; p = 0.8, Wilcoxon signed-rank test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)