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. 2023 Mar 26;72:101713. doi: 10.1016/j.molmet.2023.101713

Figure 7.

Figure 7

2-AGP promotes a time-dependent neurite retraction of POMC neurons. (A) Time-lapse fluorescence microscopy of in vitro cultured POMC-eGFP primary neurons showing the effect of 2-AGP, added in the cell medium, on the retraction of neurites (arrows). Scale bar: 20 μm. (B) Representative images showing a 30 min and 60 min time course effect of leptin, OX-A, and 2-AGP on the morphometric changes of the neuronal processes of POMC cultured neurons (range ± 2 mm) respect to the start time (t = 0) represented by the vertical dotted line. (C, D) High magnification of the cell indicated by the white dotted box area at 60 min of 2-AGP treatment showing pT231-Tau/LPA1-R co immunolabeling at the neurite indicated in the red dotted boxed (left image), (Scale bar: 10 μm). (E) Representative immunoblots of PSD95 and β-actin showing the effect of 2-AGP and AM095 on the postsynaptic density after 2-AGP treatment in wt or ob/ob mice. OD values are expressed as the ratio between total protein signal and β-actin. Data are mean ± SEM from n = 3 mice/group; p values are obtained via two-way ANOVA with Tukey's multiple comparisons test. (F) Representative confocal images showing α-MSH/PSD-95 colocalizing immunoreactivities at Hcrt-eGFP or OX-eGFP neurons of mice treated with 2-AGP. Data are from n = 6 mice/group. Scale bar = 30 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)