Figure 3.

Ehm2/1 suppresses cell migration and invasion in vitro. (A) Ehm2/1 protein levels were analyzed in Ehm2/1 overexpressed and knocked down MCF-7 cells using western blotting. (B) The mRNA expression levels of Ehm2/1 were analyzed in Ehm2/1 overexpressed and knocked down MCF-7 cells using Quantitative reverse transcription PCR (qRT-PCR). Cell migration assay and cell invasion assay were performed in Ehm2/1 overexpressed (C) and knocked down MCF-7 cells (D). Results were from a representative experiment performed in triplicate. Image J was used to perform quantitative analysis. Data were shown as means ± SD. P values were calculated by Student’s t-test. *P <0.05, **P < 0.01.