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. 2023 Apr 19;11:e15172. doi: 10.7717/peerj.15172

Figure 2. Sema4D silencing decrease PD-L1 expression.

Figure 2

B16-F10R Cell were derived into Sema4D-shRNA group and Sema4D-NC group, co-cultured with TIL at a 1:5 ratio and nivolumab 50 ng/ml 24 h, collect the cell to detect the expression of PD-L1. (A) Correlation between PD-L1 and Sema4D (B) Protein bands of PD-L1and Sema4D. (C) Ratio to GAPDH of Sema4D, p = 0.0002. (D) Ratio to GAPDH of PD-L1, p = 0.0016. (E) mRNA expression of PD-L1. Compared with Sema4D-NC group, ∗∗ is p < 0.01, ∗∗∗ is p < 0.001. The experiment was repeated three times, n = 3, t test was used for statistical analysis.