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. 2023 Apr 19;11:e15172. doi: 10.7717/peerj.15172

Figure 4. Sema4D deficiency potentiates nivolumab inhibitory effects on B16-F10R cell invasion and migration.

Figure 4

B16-F10R cells were derived into Sema4D-shRNA group and Sema4D-NC group, co-cultured with TIL at a 1:5 ratio and nivolumab 50 ng/ml, cell invasion and migration were detected. (A) Cell migration. Representative image of cell migration in the absence and presence of shRNA Sema4D at 0 h, 24 h, and 48 h. The scratched areas were measured in three random fields in each group. Wound healing analysis showed a significant difference in the cell-free area of Sema4D-shRNA group was significantly wider than that of Sema4D-NC group at 24 and 48 h (P < 0.0001). (B) Ratio of cell free area. (C) Chamber invasion of cells. Chamber invasion analysis showed a significant difference lower number of invasive cells in Sema4D-shRNA group than Sema4D-NC group (× 100) at 48 h. (D) cell invasion number per field. Compared with Sema4D-NC group,∗∗∗∗ is p < 0.0001. The expression was repeated three times, n = 3.