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. 2023 Apr 19;11:e15172. doi: 10.7717/peerj.15172

Figure 5. Sema4D knockdown is significantly associated with inhibition of PI3K-AKT signaling pathway.

Figure 5

(A) Association of clustered modules with Sema4D expression. The brown module was identified as the most relevant module for Sema4D expression. (B) Module-trait associations. (C) Gene set variation analysis revealed several significantly dysregulation malignancies events between different Sema4D expression population. (D) Protein-protein interactions of Sema4D. (E) Pathway enrichment analysis showed that Sema4D is involved in the PI3K-AKT signaling pathway. (F) PI3K-AKT signaling pathway proteins expression after Sema4D knockdown. B16-F10R cells were derived into Sema4D-shRNA group and Sema4D-NC group, co-cultured with TIL at a 1:5 ratio and nivolumab 50 ng/ml, PI3K, AKT protein expression were detected. The experiment was repeated three times, n = 3. Western blotting showed that Sema4D was significantly associated with inhibition of PI3K-AKT signaling pathway . Compared with Sema4D-NC group, ∗∗ is p < 0.01.