Table 3.

Physicochemical properties and model quality statistics of the four proteins of mpox virus identified through subtractive proteomics.

Features	Core protease I7	DNA topoisomerase 1B	Early transcription factor 70 kDa small subunit	DNA polymerase processivity factor
Gene Name	I7L	TopIB	VETFS	A20R
Enzyme Commision (EC)	3.2.22.-	5.99.1.2	3.6.4.13	3.-
KEGG Orthology (KO) Identifier	–	–	–	K21082
Closest Crystal Structure	–	2H7G (97.77%, 100%))	7AMV (99.69%, 100%)	6ZXP (96.75%, 28%)
Length (Amino Acid)	423 aa	314 aa	637 aa	426 aa
Mol. Weight (Dalton)	49023.57	36665.56	73844.91	49147.14
pI	7.85	9.50	6.93	5.62
Instability Index	33.81	40.22	34.45	36.35
Aliphatic Index	83.14	88.03	97.85	92.77
GRAVY	−0.22	−0.32	−0.17	−0.26
Predicted IDDT Score	0.64	0.87	0.70	0.80
Overall Quality Score	95.42	95.75	95.66	91.87
MolProbity Score	1.44 (96th percentile)	0.90 (100th percentile)	1.40 (97th percentile)	1.37 (98th percentile)
Clash Score	3.49 (97th percentile)	1.53 (99th percentile)	2.78 (98th percentile)	2.88 (98th percentile)
Poor Rotamers (%)	0	0	0	0
RamaPlot Most Favoured (%)	95.72 (403 aa)	100.00 (312 aa)	95.12 (604 aa)	95.80 (406 aa)
RamaPlot Allowed (%)	3.8 (16 aa)	0	3.48 (22 aa)	4 (17 aa)
RamaPlot Disallowed (%)	0.48 (2 aa)	0	1.42 (9 aa)	0.24 (1 aa)