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. 2023 Apr 22;6:445. doi: 10.1038/s42003-023-04821-2

Fig. 4. ETS1 regulates the genome-wide binding of p52.

Fig. 4

a DNA binding dynamics of p52 in TWEAK-treated U-87 MG cells following ETS1 KD. Venn diagram depicting b the number of cooperative sites between p52 and ETS1, and c the number of p52:ETS1 co-binding sites lost following ETS1 KD. d RNA-seq tracks and p52, ETS1 and RNA pol II ChIP-seq tracks at the ERN1 promoter following NF-κB/p52 activation as well as NFKB2 and ETS1 KD. Genomic changes observed at the ERN1 promoter are marked in a red box. GRCh38 assembly was used. e p100/p52, ETS1 and IRE1α protein expression analysed by western blotting in U-87 MG and U-251 MG cell lines following NF-κB/p52 activation as well as NFKB2 and ETS1 KD. The western blot involving U-251 MG cells was run over two blots at the same time and loaded with the same amount of protein. The proteins probed on each blot are indicated under Blot 1 and Blot 2.