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. 2023 Apr 22;6:445. doi: 10.1038/s42003-023-04821-2

Fig. 5. p52:ETS1 cooperativity promotes glioma cell invasion and proliferation through IRE1α.

Fig. 5

a IRE1α protein expression analysed by western blotting to validate shRNA-mediated repression of IRE1α in U-87 MG and U-251 MG cells. b Plot depicts the average number of cells relative to 0 h (mean ± s.d) in U-87 MG and U-251 MG shIRE1α. c Transwell invasion assay was performed in U-87 MG and U-251 MG shIRE1α cells. Representative images from n = 3 biological replicates are shown. Scale bars: 400 µm. Plots (right) depict the average number of invaded cells (mean ± s.d) across five fields. All functional experiments were compared in the presence and absence of TWEAK treatment. Two-way ANOVA was used for all statistical analyses. *P < 0.05; **P < 0.01; ***P < 0.001.