Table 2.
Summary of O2 only study characteristics
| Author (y) | Country | Animals studied | Oxygen studied | Measures reported | ||||
|---|---|---|---|---|---|---|---|---|
| Type (Strain) | Sex | Age | Wgt | FiO2 | Duration | |||
|
Hackney (1975) |
US | Monkey (Squirrel) | M | NR | NR | 0.21, 0.60, 0.80 | 2, 4, 8d | Lung injurya, lung or systemic immune response parametersb |
| Hayatdavoudi (1981) | US | Rat (Charles River – CD) | M | NR | 300–350 g | 0.21, 0.60, 0.85 | 7d | Body weights; lung injury; lung or systemic immune response parameters; survival with FiO2 = 1.0 after prior lower FiO2 exposure |
|
Rister (1983) |
Germany | Guinea pigs | NR | NR | NR | 0.21, 0.30, 0.40, 0.50, 0.60, 0.70, 0.80 | 0, 18, 42, 60, 90 h | Lung injury |
|
Coursin (1987) |
US |
Rats (SD) |
M | NR | 160–200 g | 0.21, 0.50, 0.65, 0.80 | 7, 14, 21, 28, 42d | Body weights; lung injury; lung or systemic immune response parameters; Survival with FiO2 = 1.0 after prior lower FiO2 exposure |
|
Holm (1987) |
US | Rabbits (NZW) | M | NR | 1.9–2.2 kg | 0.21, 0.60 | 21d | Lung injury; lung or systemic immune response parameters |
|
Nickerson (1990) |
US |
Rabbits (NZW) |
NR | NR | 2.0–3.0 kg | 0.21, 0.60 | 21d | Lung injury |
|
Nylen (1993) |
US |
Hamsters (Syrian Gold) |
M | 6weeks | NR | 0.21, 0.60 | 7, 21, 90d | Body weight; lung injury |
| Van Klaveren (1997) | Belgium, UK | Rats (Wistar) | M | NR | NR | 0.21, 0.60, 0.85 | 7d | Body weight; lung injury; lung or systemic immune response parameters; survival |
|
Belik (2003) |
Canada |
Rats (SD) |
F | NR | 250–275 g | 0.21, 0.60 | 14d | Lung injury |
|
Nelin (2003) |
US |
Rats (SD) |
M | NR | 250–325 g | 0.21, 0.50, 0.90 | 2.5d | Body weight; lung injury; salicylate conversion to 2,3-DHBA to assess lung hydroxyl radical oxidant production |
|
Hesse (2004) |
Germany |
Mice (C57BL6/J) |
M | 12–16 wk | 26–27 g | 0.21, 0.60, > 0.95 | 3d | Body weight; lung injury; lung or systemic immune response parameters; body weight; survival |
|
Gan (2011) |
US |
Rats (SD) |
M | NR | 275–325 g | 0.21, 0.60, 0.85 | 7d | Body weight; lung injury; lung or systemic immune response parameters |
|
Audi (2012) |
US |
Rats (SD) |
M | NR | 300–325 g | 0.21, 0.60, 0.95 |
60–7d 95–2d |
Lung injury; lung or systemic immune response parameters |
|
Lagishetty (2014) |
US |
Mice (C57BL/6 J) |
M | 9 weeks | NR | 0.21, 0.50, 0.75, 1.0 | 3d | Lung injury; lung or systemic immune response parameters; lung tissue expression of 6 CLOCK proteins (CLOCK, Bmal1, Cry1, Cry2, Per1, Per2) |
F female, M male, MAC macrophage, NR not reported, NZW New Zealand White, SD Sprague–Dawley, UC unclear, US United States, UK United Kingdom, Wgt weight, Bmal1—CLOCK circadian locomotor output cycles kaput, 2,3-DHBA dihydroxy benzoic acid, GSH glutathione, HMPAQ Tc labele3d hexamethylpropyleneamine oxide (trapped in the lung by GSH), DEM GSH depleter diethyl maleate, GSH-glutathione GSSG-oxidized glutathione
aLung injury measures included one or more of the following: arterial oxygen pressure; lung weights, lung wet to dry weight ratios, lung to body weight ratios; bronchoalveolar lavage (BAL) volume, cell, protein or albumin concentrations; alveolar permeability to solute; lung pressure/volume relationships, lung volumes, diffusion capacity; lung histologic changes; pulmonary artery and airway contractile and relaxation function, and/or alveolar macrophage and neutrophil microtubule and microfilament integrity, BAL type-2 cell dysfunction or phospholipid content
bLung or systemic immune response parameters included one or more of the following: BAL cellularity and polymorphonuclear neutrophil cell numbers, BAL or lung tissue macrophages, BAL or lung tissue lymphocytes; BAL or serum cytokine, nitric oxide, or nitric oxide synthase levels; lung antioxidant activity which included HMPAQ retention with or without DEM based on lung to background ratio, lung or BAL glutathione peroxidase (GPx), glutathione (GSH), oxidized glutathione (GSSG), gamma-glutamyltransferase, non-protein-sulfhydral (NPSH), and/or superoxide dismutase (SOD) levels