Fig. 4. Effect of puerarin in combination with canagliflozin on oxidative stress and the inflammatory response of kidneys.

(A) MDA level in the kidney. (B) GPx activity in the kidney. (C) SOD activity in the kidney. (D) CAT activity in the kidney. (E) GSH levels in the kidney. (F) Western blot of TNFα, IFNγ, IL6, and IL1β in the kidney. (G) Relative expression of TNFα. (H) Relative expression of IFNγ. (I) Relative expression of IL6. (J) Relative expression of IL1β. Data are shown as mean ± SD (n = 6). MDA, malondialdehyde; GPx, glutathione peroxidase; SOD, superoxide dismutase; CAT, catalase; GSH, glutathione; TNFα, tumor necrosis factor α; IFNγ, interferon γ; IL, interleukin. ^#p < 0.05, ^##p < 0.01 vs. control group; *p < 0.05, **p < 0.01 vs. diabetes group.