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AlaRS charges L-Ala contaminant in D-alanylation reaction. (A) ESI-MS profile of resultant product after aminoacylated by EcAlaRS using D- and L-alanine. (B) Chromatograms showing that area of the peak corresponding to L-alanine in D-alanine sample is increasing upon titration of increasing concentration of D-alanine in the reaction.
