Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 17;51(7):3465–3484. doi: 10.1093/nar/gkad165

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 4. — Genome editing combining regular SN plasmid donors or modified ITPN donors and nicking orthogonal SaCas9 complexes. SaCas9^D10A- or SaCas9^N580A-dependent genome editing at AAVS1 and CLYBL loci in HeLa cells using EGFP-encoding donors, and at these loci in iPSCs using Puro^R.EGFP-encoding donors, was assessed by reporter-directed flow cytometry and colony-formation assays, respectively. The latter assay detected the pluripotency marker alkaline phosphatase to identify puromycin-resistant iPSCs. Controls consisted of cells exposed to regular donor plasmids and nickases lacking locus-specific gRNAs. Data are shown as mean ± SD of at least three independent biological replicates. Significant differences between the indicated datasets were calculated by two-tailed unpaired Student's t tests (left and middle graphs) and two-tailed paired ratio t test (right graph); ***0.0001 < P< 0.001, **0.001 < P< 0.01, *0.01 < P< 0.05.