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. 2023 Feb 10;51(7):3116–3129. doi: 10.1093/nar/gkad068

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Search for the chromosomal integration site attB by ICEBs1. First column: snapshots of ICEBs1 labelled with GFP in transconjugant cells at the indicated times (green dots), ∼1 h after mating of MMB1277 as a donor and SAM318 as a recipient (Materials and Methods), with corresponding to the time at which the first image was recorded; second column: attB labelled with mApple2 (red dots); third column: overlay of GFP (ICEBs1) and mApple2 (attB) images; fourth column: overlay of third column with phase contrast images, showing the presence of non-fluorescent donor cells. Arrows show a moving ICEBs1 that integrates (see also Movie S1). The scale bar represents 1 μm.

Inline graphic — Search for the chromosomal integration site attB by ICEBs1. First column: snapshots of ICEBs1 labelled with GFP in transconjugant cells at the indicated times (green dots), ∼1 h after mating of MMB1277 as a donor and SAM318 as a recipient (Materials and Methods), with corresponding to the time at which the first image was recorded; second column: attB labelled with mApple2 (red dots); third column: overlay of GFP (ICEBs1) and mApple2 (attB) images; fourth column: overlay of third column with phase contrast images, showing the presence of non-fluorescent donor cells. Arrows show a moving ICEBs1 that integrates (see also Movie S1). The scale bar represents 1 μm.