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. 2023 Feb 10;51(7):3116–3129. doi: 10.1093/nar/gkad068

Table 1.

B. subtilis strains used

Strain Relevant genotypea (comments) Fluorescence
SAM318 (recipient) ICEBs10 (cured of ICEBs1) ΔyddS::(tetO240 cat; near attB) ΔcgeD::{Ppen(mutTAGG)-tetR-mApple2 tet} ΔamyE::(Ppen-lacI-gfpmut2 spc) GFP (foci if ICEBs1+); mApple2 (attB)
MMB1277 (donor) ICEBs1 Δ(rapI-yddM)::{lacO (arrays in ICEBs1) kan} amyE::(Pxyl-rapI spc) None
SAM837 ICEBs1 Δ(rapI-yddM)::{lacO (arrays in ICEBs1) kan} ΔyddS::(tetO240 cat; near attB) ΔcgeD::{tet Ppen(mutTAGG)-tetR-mApple2} ΔamyE::(Ppen-lacI-gfpmut2 spc) GFP (ICEBs1); mApple2 (attB)
SAM049 ΔcgeD::{tet Ppen(mutTAGG)-tetR-mApple2} 300°::(tetO kan) mApple2 (300°)

aAll strains contain the trpC2 pheA1 alleles (not indicated).