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. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Andrology. 2022 Nov 8;11(5):789–798. doi: 10.1111/andr.13329

Figure 2: Fertilizing ability and phenotypic analysis of Pdcl2−/− male mice.

Figure 2:

(A) Average number of pups born per delivery. N=4 males each for Pdcl2+/− and Pdcl2−/−, mated with two females per male. ***P<0.001, Student’s t-test.

(B) Testis to body weight ratio. N=3 males for wild-type and N=4 Pdcl2−/−. **P<0.01, Student’s t-test.

(C) PAS and hematoxylin staining of testicular sections from wild-type and Pdcl2−/− mice. Scale bars: 50 μm.

(D) Sperm motility results using computer-assisted sperm analysis (CASA). ***P<0.001, Student’s t-test.

(E) Observation of the acrosome and flagella using EGFP expressed under the Acrosin promoter and mitochondria-targeted DsRed2 in the principal piece in wild-type and Pdcl2−/− sperm. Scale bars: 10 μm.