(Opposite and above) Cytoskeletal RNAs. (A) Schematic representation of portions of the TM, TP and AC genes. Transcripts generated in this study are represented by stippled boxes. Hatched boxes mark the PCR primers used in amplifying the sequences from a cDNA library. (B) Secondary structures of the transcripts predicted by MFold (GCG) web-based sequence analysis program. (C) Integrity of the TM, TP, AC and polylinker RNAs. The RNAs were synthesized in vitro using T7 RNA polymerase, purified, and 200 ng of each was loaded onto a 1% agarose gel. Positions of RNA markers (Gibco BRL) are indicated.