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. 2023 Mar 2;64(4):100351. doi: 10.1016/j.jlr.2023.100351

Fig. 1.

Fig. 1

Loss and overexpression of SGPL1 results in a perturbance of sphingolipid species levels and significant changes in growth and migration. A: Schematic of the sphingolipid pathway where SGPL1 performs the final degradative process. B: SGPL1_Control shows a detectable band at 60 KDa, SGPL1_KO show no detectable band and SGPL1_OE show endogenous SGPL1 at 60 KDa and a band at 90 KDa representing SGPL1 linked to GFP. C: Proliferation rate measured of cells grown in serum-free media by MTT assay after 96 h. D, E: Levels of intracellular and extracellular sphingolipid intermediates in SGPL1_Control, SGPL1_KO and SGPL1_OE grown in FBS-containing media. F: Migration assay and quantification of wound gap closure (%) of cell grown in FBS media. (All statistical analyses were conducted using one-way ANOVAs with Tukey’s multiple comparison test, n = 3. Scale bar = 500 μm). G: Proliferation rates measured by MTT assay of cells grown in FBS after 96 h. SGPL1, Sphingosine 1-phosphate lyase.