Fig. 4.

SGPL1_KO models present with a thickened stratum corneum and increased differentiation. A: Representative H&E-stained images of SGPL1_Control and SGPL1_KO 3D organotypic models. Total epidermal and stratum corneum thickness were measured in μm using ImageJ. Scale bar = 50 μm. B: Immunofluorescent staining of organotypic models for differentiation markers KRT14, KRT10, involucrin, loricrin, and filaggrin. Images were captured at the same exposure and gain for comparison. Scale bar = 50 μm. (Statistical analysis was conducted using unpaired t test, n = three-fourths, ∗P < 0.05.). SGPL1, Sphingosine 1-phosphate lyase.